■ 基本信息
启动子: | T7 |
复制子: | ColA |
终止子: | T7 terminator |
质粒分类: | 大肠杆菌载体；双表达框质粒 |
质粒大小: | 3719bp |
质粒标签: | N-6×His,C-S |
原核抗性: | Kan |
克隆菌株: | DH5a |
培养条件: | 37℃，有氧，LB |
表达宿主: | BL21(DE3) |
诱导方式: | IPTG或乳糖及其类似物 |
5'测序引物: | ACYCDuetUP1：GGATCTCGACGCTCTCCCT DuetUP2:TTGTACACGGCCGCATAATC |
3'测序引物: | DuetDOWN1:GATTAGCGGCCGTGTACAA T7-ter:TGCTAGTTATTGCTCAGCGG |
备注: | 同时表达两个外源蛋白 |
■ 质粒属性
质粒宿主: | 大肠杆菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: | 多框空载
|
原核抗性: | Kan |
真核抗性: |
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荧光标记: |
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■ 质粒简介
pCOLADuet-1 is designed for the coexpression of two target genes from a single plasmid. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). MCS-1 encodes the six-amino acid His•Tag sequence for the creation of a N-terminal fusion and MCS2 encodes the 15 amino acid S•Tag™ peptide after the last restriction site for the creation of a C-terminal fusion if desired. Genes inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. The vector has the COLA replicon from ColA(1) and the kanamycin resistance gene. This vector can be transformed into the same cell with plasmids containing compatible origins of replication and drug resistance genes for coexpression of up to 8 target genes.
质粒只保证关键序列正确，不保证表达效果。
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK325pCOLADuet-1大肠双表达质粒.txt
