■ 基本信息

■ 质粒属性

■ 质粒简介

pCDNA3.0载体是专门设计用来在哺乳细胞中进行高水平的稳定表达和瞬时表达。其中人巨细胞病毒早期启动子（CMV）在广泛的哺乳动物细胞中可以实现高表达。另外为筛选稳定细胞系提供了新霉素抗性基因，可用G418筛选。

pcDNA3.0 is 5.4 kb vectors  designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.  Neomycin resistance gene for selection of stable cell lines.

pcDNA3.0 vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin selectable marker and a forward-orientation multiple cloning site. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells Multiple cloning sites orientations to facilitate cloning .Neomycin resistance gene for selection of stable cell lines Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen.

特征片段：

                       CMV promoter: bases 209-863                                    T7 promoter: bases 864-882

                       Polylinker: bases 889-994                                            Sp6 promoter: bases 999-1016

                       BGH poly A: bases 1018-1249                                     SV40 promoter: bases 1790-2115

                       SV40 origin of replication: bases 1984-2069                Neomycin ORF: bases 2151-2945

                       SV40 poly A: bases 3000-3372                                    ColE1 origin: bases 3632-4305

                       Ampicillin ORF: bases 4450-5310

质粒只保证关键序列正确，不保证表达效果。

■ 质粒图谱

■ 质粒序列

质粒序列请下载：ZK344pCDNA3.0哺乳表达质粒.txt