■ 基本信息
别名: | pCDNA3.1+ |
启动子: | CMV |
复制子: | pUC |
终止子: | BGH poly(A) signal |
质粒分类: | 哺乳系列质粒；哺乳表达质粒；pCDNA系列质粒 |
质粒大小: | 5428bp |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培养条件: | 37℃，5%CO2 |
表达宿主: | 293T等哺乳细胞 |
诱导方式: | 无需诱导 |
5'测序引物: | pCDNA3.1-F（CTAGAGAACCCACTGCTTAC） |
3'测序引物: | pCDNA3.1-R（TAGAAGGCACAGTCGAGG） |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: | G418
|
荧光标记: |
|
■ 质粒简介
pCDNA3.1(+)是来自pCDNA3的5.4kb载体，设计用于哺乳动物宿主中的高水平稳定和瞬时表达。大多数哺乳动物细胞可以进行高水平的稳定和非复制性瞬时表达。人类巨细胞病毒立即早期（CMV）启动子，用于在广泛哺乳动物细胞中的高水平表达。前向（+）和反向（-）方向上的多个克隆位点，以促进克隆用于选择稳定细胞系的新霉素抗性基因。在潜伏感染SV40或表达SV40大T抗原（例如COS-1，COS-7）的细胞系中的异常复制。质粒只保证关键序列正确，不保证表达效果。
pcDNA3.1(+) and pcDNA3.1(-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells
• Multiple cloning sites in the forward (+) and reverse (-) orientations to facilitate cloning
• Neomycin resistance gene for selection of stable cell lines
• Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). The control plasmid, pcDNA3.1/CAT, is included for use as a positive control for transfection and expression in the cell line of choice.
This pcDNA3.1(+)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site.
pCDNA3.1(+)的参考文献：Clinical significance of bromodomain containing protein 7 and its association with tumor progression in prostate cancer.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK347pCDNA3.1(+)哺乳表达质粒.txt
