■ 基本信息
启动子: | CMV |
复制子: | pUC |
终止子: | BGH poly（A）signal |
质粒分类: | 哺乳系列质粒；哺乳表达质粒；pCDNA系列质粒 |
质粒大小: | 5541bp |
质粒标签: | N-3×HA |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培养条件: | 37℃，5%CO2 |
表达宿主: | 293T等哺乳细胞 |
诱导方式: | 无需诱导 |
5'测序引物: | pCDNA3.1-F（CTAGAGAACCCACTGCTTAC） |
3'测序引物: | pCDNA3.1-R（TAGAAGGCACAGTCGAGG） |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: | G418
|
荧光标记: |
|
■ 质粒简介
pCDNA3.1-3×HA-N质粒是一种高效表达的哺乳质粒。该质粒含有Amp抗性，可转至DH5α中转化。培养条件为LB培养基，37℃。
pCDNA3.1-3×HA-N vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin selectable marker and a forward-orientation multiple cloning site.High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells Neomycin resistance gene for selection of stable cell lines. There are three HA tags on the N side.Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen.
质粒只保证关键序列正确，不保证表达效果。
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK354pCDNA3.1-3×HA-N哺乳表达质粒.txt
