■ 基本信息
别名: | Plasmid #22733 |
启动子: | PGK |
复制子: | ori，F1 ori |
终止子: | bGH poly(A) signal |
质粒分类: | 基因文库质粒；其它基因质粒；其它种源质粒 |
质粒大小: | 6678bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | DH5a |
培养条件: | 37度 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: | Puro
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荧光标记: |
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■ 质粒简介
This vector was designed for assembling gene targeting constructs using the BAC recombineering approach. The vector contains a puromycin-(delta)thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pUDTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo). These selectable markers are flanked by lox66 and lox2272 sites (Cre-recombinase recognition sequences) in a pBluescript KS+ backbone with a modified multi-cloning region.
pLCA.66/2272质粒的参考文献：Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK1548 pLCA.662272同源重组质粒.txt
