■ 基本信息
别名: | pBAD-18 |
启动子: | araBAD |
复制子: | pBR322 |
质粒分类: | 大肠杆菌载体；pBAD系列表达质粒 |
质粒大小: | 4613bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37℃，有氧，LB |
表达宿主: | 推荐LMG194 |
诱导方式: | 阿拉伯糖 |
5'测序引物: | pBAD-F: ATGCCATAGCATTTTTATCC |
3'测序引物: | pBAD-R: GATTTAATCTGTATCAGG |
■ 质粒属性
质粒宿主: | 大肠杆菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 空载体 |
原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
This vector is one of several tightly controlled expression vectors regulated by the arabinose operon. Vectors differ in replicon, antibiotic resistance marker, multiple cloning site and need for translation initiation sequences. Cultures should be grown in minimal media for more reproducible induction of expression. Expression is induced in glycerolcontaining media by the addition of arabinose. Expression is repressed by addition of glucose or other catabolites. Cloned inserts must provide a translation initiation sequence (ATG) and ribosome binding site for expression. Expression of cloned inserts in the two recommended hosts differ in terms of induction characteristics, especially when using low concentrations of inducer (arabinose) and growing in minimal media. The following primers can be used for sequencing of cloned inserts: 5 primer (278bp upstream of the NheI site) :5 CTGTTTCTCCATACCCGTT 3and one of two 3 primers:3 primer 1 (219bp downstream of the HindIII site):5 CTCATCCGCCAAAACAG 33 primer 2 (1733bp downstream of the HindIII site):5 GGCTGAAAATCTTCTCT3
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK1604 pBAD18大肠表达质粒.txt
